In vitro observations on the influence of copper peptide aids for the LED photoirradiation of fibroblast collagen synthesis

Huang PJ, Huang YC, Su MF, Yang TY, Huang JR, Jiang CP · Photomedicine and Laser Surgery

Adding Cu-GHK after irradiation raised procollagen I C-peptide production by about 30%, COL1 mRNA by about 70% and bFGF secretion by about 230% relative to LED alone, with a dose-response across LED energy.

Population

HS68 human dermal fibroblasts

Intervention

Red LED photoirradiation at 0, 1 or 2 J followed by Cu-GHK incubation

Comparator

LED photoirradiation alone

Limitations

One cell line, one laboratory; a combination protocol, so the peptide's independent contribution is bounded by the LED-alone arm only.