Thymosin beta 4 induces colon cancer cell migration and clinical metastasis via enhancing ILK/IQGAP1/Rac1 signal transduction pathway

Tang MC, Chan LC, Yeh YC, Chen CY, Chou TY, Wang WS, Su Y · Cancer Letters

Overexpression raised Rac1 activity and IQGAP1/ILK complex levels and increased migration; knocking Tβ4 down lowered both proteins and the cells' migratory capacity, with the same pattern seen in patient tissue.

Population

Tβ4-overexpressing SW480 colon cancer cells; patient tumour immunohistochemistry

Intervention

Tβ4 overexpression and knockdown

Comparator

Parental and non-targeting-knockdown cells

Limitations

Single cell line, one research group; the patient component is immunohistochemical correlation.