Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA

Fedoreyeva LI, Kireev II, Khavinson VKh, Vanyushin BF · Biochemistry (Moscow)

Labelled short peptides entered the cytoplasm and the nucleus of HeLa cells and bound particular oligonucleotide sequences in vitro — the experimental basis for the program's claim that these peptides act directly on DNA.

Population

HeLa cells and synthetic deoxyribooligonucleotides

Intervention

Fluorescence-labelled short peptides of the Khavinson series

Comparator

Unlabelled and control sequences

Limitations

Cancer cell line; binding and localisation, not a demonstrated change in transcription of a named gene; Khavinson is a co-author, though the work was done at the All-Russia Research Institute of Agricultural Biotechnology in Moscow.