Sulforaphane inhibits proliferation by causing cell cycle arrest at the G2/M phase in rabbit articular chondrocytes

Jeong HJ, Yu SM, Jung JC, Kim SJ · Molecular Medicine Reports

Arrested chondrocyte proliferation at G2/M: 50 μM cut cell numbers by 45% at 24 hours and lifted the G2/M fraction from 12% to 37%, through lower cyclin B1, cdc2 and cdc25c and higher p21 and p53. The cell-cycle effects that make sulforaphane interesting in cancer research are not obviously wanted in a tissue that repairs slowly and has few cells to spare.

Population

Rabbit articular chondrocytes

Intervention

Sulforaphane at up to 50 μM for 24 or 48 hours

Comparator

Untreated chondrocytes

Limitations

Chondrocytes from 2-week-old rabbits, concentrations well above plasma levels, no functional cartilage endpoint.