Doping control analysis of TB-500, a synthetic version of an active region of thymosin β4, in equine urine and plasma by liquid chromatography-mass spectrometry

Ho EN, Kwok WH, Lau MY, Wong AS, Wan TS, Lam KK, Schiff PJ, Stewart BD · Journal of Chromatography A

Confirmed Ac-LKKTETQ and its metabolites in post-administration equine plasma and urine down to 0.02 and 0.01 ng/mL — the first published detection of administered TB-500 in a living animal, and the basis for the doping-control assays now in routine use.

Population

Horses given a single dose of a veterinary TB-500 preparation

Intervention

Single dose of TB-500 containing 10 mg N-acetylated LKKTETQ

Comparator

Pre-administration samples

Limitations

Analytical study in horses; measures detectability, not effect, and carries no efficacy or safety information.