[POLYPEPTIDES INFLUENCE ON TISSUE CELL CULTURES REGENERATION OF VARIOUS AGE RATS]

Ryzhak AP, Chalisova NI, Lin'kova NS, Khalimov RI, Ryzhak GA, Zhekalov AN · Advances in Gerontology (Uspekhi Gerontologii)

Each preparation raised the growth-zone area index of its own source tissue and essentially nothing else. In cartilage, Chondrolux gave 37.5±3.1% in explants from young rats and 31.3±5.1% in old ones, both p<0.05, while the other six preparations ranged from 0.2% to 5.0% there and none reached significance. PCNA rose and p53 fell in the matching tissues. One cross-tissue effect was significant and negative rather than absent: the prostate preparation suppressed the pineal growth zone by 21.5±3.8% in young rats.

Population

300 organotypic explants of each of seven tissues — brain cortex, pineal, liver, prostate, spleen, myocardium and xiphoid-process cartilage — from 3-month-old and 24-month-old Wistar rats

Intervention

Seven bovine-tissue polypeptide preparations screened at 0.01–100 ng/ml, with 20–50 ng/ml effective, including Chondrolux (from cartilage), the closest indexed sibling of Sigumir

Comparator

Untreated explants of the same tissue, taken as the zero line for the area index

Limitations

A tissue-specificity result and not a repair or matrix one: the endpoint is the ratio of the whole explant's area to its central zone, with immunocytochemistry beside it, and nothing here measures matrix or load. The cartilage is xiphoid process rather than articular. Chondrolux is a sibling preparation, not Sigumir itself. The paper's reading of high selectivity sits a little awkwardly with the one significant cross-tissue suppression in its own table. Institute-authored, in a Russian-language journal tied to that institute.