A Polyphenol-rich Pomegranate Fruit Extract Suppresses NF-κB and IL-6 Expression by Blocking the Activation of IKKβ and NIK in Primary Human Chondrocytes

Haseeb A, Khan NM, Ashruf OS, Haqqi TM · Phytotherapy Research

Suppressed IL-6 at mRNA and protein level and reduced reactive oxygen species, by blocking IKKβ phosphorylation, IκBα degradation and NF-κB/p65 nuclear translocation — and also NF-κB-inducing kinase, which places the effect on both the canonical and non-canonical arms of the pathway.

Population

Primary human chondrocytes stimulated with IL-1β

Intervention

Polyphenol-rich pomegranate fruit extract

Comparator

IL-1β alone

Limitations

Cell culture, same laboratory as the rabbit and MAPK work.