Enhanced biological properties of collagen/chitosan-coated poly(ε-caprolactone) scaffold by surface modification with GHK-Cu peptide and 58S bioglass

Molavi AM, Sadeghi-Avalshahr A, Nokhasteh S, Naderi-Meshkin H · Progress in Biomaterials

Every surface supported cell attachment and none was cytotoxic over seven days. GHK-Cu raised fibroblast viability against the uncoated scaffold at day 3 only, with no difference at days 5 or 7; the day-7 improvement belongs to the two bioactive-glass surfaces, which showed nothing at day 5. The uncoated scaffold, the GHK-Cu surface and the lower-concentration bioactive glass all had antibacterial activity against E. coli and S. aureus at 24 hours, GHK-Cu's the greatest of the three, and GHK-Cu alone showed activity by one hour.

Population

Electrospun poly(ε-caprolactone) scaffolds coated with collagen/chitosan

Intervention

Surface modification with 1 mM GHK-Cu, or with 58S bioactive glass

Comparator

Uncoated collagen/chitosan-coated scaffold

Limitations

Materials-science characterisation with viability and antibacterial assays on fibroblasts rather than chondrocytes; no differentiation, matrix or animal endpoint, and nothing touching a joint. Funded by an academic research centre, with no conflict of interest declared.