Analysis of the Anti-Inflammatory Capacity of Bone Broth in a Murine Model of Ulcerative Colitis

Mar-Solís LM, Soto-Domínguez A, Rodríguez-Tovar LE, et al. · Medicina (Kaunas)

Bone broth reduced colonic pro-inflammatory cytokine expression (IL-6 down ~95%, IL-1beta down ~61%, TNF-alpha down ~69%) and raised anti-inflammatory IL-10 and IL-4. A gut-inflammation model — not a joint or cartilage outcome. Its second contribution is compositional and is the only full amino-acid analysis of a defined bone stock in PubMed: 248.5 mg protein and 232.9 mg total amino acids per 100 mL (54.6% essential), glycine 15.0 mg/100 mL, proline 3.4, methionine 9.3, cysteine 3.5, and total minerals 25.0 mg/100 mL.

Population

24 male BALB/c mice, 6 per arm, with chemically induced ulcerative colitis, killed 24 h after induction

Intervention

Prophylactic oral bone stock (bovine femur, 8 h at 100 +/- 2 C, bone:water 1:4, 20 mL white vinegar per litre) before colitis induction

Comparator

Colitis controls without broth

Limitations

Mouse colitis model with six per arm, a 24-hour endpoint and unscored histology; single study. Hydroxyproline and hydroxylysine were not measured, so the amino acid panel cannot be used to estimate collagen content directly. Funded by Paicyt-UANL; no conflicts declared.