Effect of the tripeptide glycyl-L-histidyl-L-lysine on the proliferation and synthetic activity of chick embryo chondrocytes

Pesáková V, Novotná J, Adam M · Biomaterials

GHK raised chondrocyte proliferation and the collagen synthetic rate on both collagen supports. Which collagen the cells made tracked the support: on cartilage collagen the cells synthesised predominantly type II, on the denatured skin-collagen support types I and III. The authors proposed GHK supplementation for preparing cartilage implants.

Population

Chick embryo chondrocytes cultured on plastic, on cartilage collagens, and on denatured insoluble skin collagen (ISC-40)

Intervention

Culture medium supplemented with (Gly-His-Lys)2·Cu·2H2O·2NaCl

Comparator

Unsupplemented medium on the same three supports

Limitations

Chick embryo cells; the abstract reports directions, not effect sizes or dispersion. Support material rather than GHK governed which collagen type dominated. No aggrecan, proteoglycan or mechanical endpoint.